Lime Stained Glass is a dyed variant of glass that retains transparency while displaying a lime-green tint. It is crafted by combining glass blocks with lime dye. Like regular glass, Lime Stained Glass must be broken with a Silk Touch-enchanted tool to drop itself; otherwise, it shatters. Mobs cannot spawn on it, and they perceive it as opaque, even though players can see through it.

Lime Stained Glass can be used to alter the color of beacon beams by averaging its RGB color values with other stained glass blocks stacked above the beacon. Additionally, it serves as a crafting ingredient for Lime Stained Glass panes.